西北农林科技大学 植物保护学院

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An expanded subfamily of G-protein-coupled receptor genes in Fusarium graminearum required for wheat infection

作者:   来源:   发布日期:2019-06-14  浏览次数:

  论文信息:Cong Jiang   , Shulin Cao , Zeyi Wang , Huaijian Xu , Jie Liang , Huiquan Liu , Guanghui Wang ,Mingyu Ding , Qinhu Wang , Chen Gong , Chanjing Feng , Chaofeng Hao and Jin-Rong Xu*.An expanded subfamily of G-protein-coupled receptor genes in Fusarium graminearum required for wheat infection. NAtuRe MiCRobioLoGy.

  JCR分区Q1,中科院大类一区,IF= 14.174

  论文摘要:The cAMP–PKA and MAP kinase pathways are essential for plant infection in the wheat head blight fungus Fusarium graminearum. To identify upstream receptors of these well-conserved signalling pathways, we systematically characterized the 105 G-protein-coupled receptor (GPCR) genes. Although none were required for vegetative growth, five GPCR genes (GIV1–GIV5) significantly upregulated during plant infection were important for virulence. The giv1 mutant was defective in the formation of specialized infection structures known as infection cushions, which was suppressed by application of exogenous cAMP and dominant active FST7 MEK kinase. GIV1 was important for the stimulation of PKA and Gpmk1 MAP kinase by compounds in wheat spikelets. GIV2 and GIV3 were important for infectious growth after penetration. Invasive hyphae of the giv2 mutant were defective in cell-to-cell spreading and mainly grew intercellularly in rachis tissues. Interestingly, the GIV2–GIV5 genes form a phylogenetic cluster with GIV6, which had overlapping functions with GIV5 during pathogenesis. Furthermore, the GIV2–GIV6 cluster is part of a 22-member subfamily of GPCRs, with many of them having in planta-specific upregulation and a com-mon promoter element; however, only three subfamily members are conserved in other fungi. Taken together, F. graminearum has an expanded subfamily of infection-related GPCRs for regulating various infection processes.

 

 

 

 

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