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Identification of two clip-domain serine proteases involved in the pea aphid’s defense against bacterial and fungal infection

作者:   来源:   发布日期:2019-04-11  浏览次数:

      论文信息:Li Ma, Feng Chen, Wen Wang, Lu Xu and Zhi-Qiang Lu*. Identification of two clip-domain serine proteases involved in the pea aphid’s defense against bacterial and fungal infection. Insect science 

       JCR分区Q1,中科院大类分区三区,IF=2.091

        论文摘要:Phenoloxidases (POs) are required for the pea aphid’s defense against bacterial and fungal infection. Prophenoloxidases (PPOs) are proteolytically converted to its active form PO through a clip-domain serine protease cascade. In this study, we identified five clip-domain serine proteases in the pea aphids. The mRNA levels of two of them, Ap_SPLP and Ap_VP were upregulated by gram-positive bacterium Staphylococcus aureus and fungus Beauveria bassiana infections. Double-stranded RNA based expression knockdown of these two genes resulted in reduced PO activity of the aphid hemolymph, higher loads of S. aureus and B.bassiana in the aphids, and lower survival rates of the aphids after infections. Our data suggest that Ap_SPLP and Ap_VP are involved in PPO activation pathway in the pea aphid.

 

       查看原文:https://onlinelibrary.wiley.com/doi/pdf/10.1111/1744-7917.12673