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论文信息：Bing Wang, Jinping Wei , Na Song , Ning Wang , Jing Zhao and Zhensheng Kang * .A novel wheat NAC transcription factor, TaNAC30, negatively regulates resistance of wheat to stripe rust.Journal of Integrative Plant Biology（2018）60（5）：432-443.
论文摘要：NAC transcription factors are widespread in the plant kingdom and play essential roles in the transcrip-tional regulation of defense responses. In this study, we
isolated a novel NAC transcription factor gene, TaNAC30,from a cDNA library constructed from wheat (Triticum aestivum) plants inoculated with the stripe rust pathogen
Puccinia striiformis f. sp. tritici (Pst). TaNAC30 contains a typical NAM domain and localizes to the nucleus. Yeast one-hybrid assays revealed that TaNAC30 exhibits tran-scriptional activity and that its C-terminus is necessary for the activation of transcription. Expression of TaNAC30 increased when host plants were infected with a virulent
race(CYR31)of the rust fungus Pst. Silencing of TaNAC30 by virus-induced gene silencing inhibited colonization of the virulent Pst isolate CYR31. Moreover, detailed histological
analyses showed that silencing of TaNAC30 enhanced resistance to Pst by inducing a significant increase in the accumulation of H 2 O 2 . Finally, we overexpressed TaNAC30
in fission yeast and determined that cell viability was severely reduced in TaNAC30-transformed cells grown on medium containing H 2 O 2 . These results suggest that TaNAC30 negatively regulates plant resistance in a compatible wheat-Pst interaction.