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论文信息：Daipeng Chen, Chunlan Wu, Chaofeng Hao, Panpan Huang, Huiquan Liu, Zhuyun Bian and Jin-Rong Xu* . Sexual specific functions of Tub1 beta‐tubulins require stage‐specific RNA processing and expression in Fusarium graminearum.Environmental Microbiology (2018) 20(11), 4009–4021.
论文摘要：The wheat head blight fungus Fusarium graminearum has two highly similar beta-tubulin genes with over-lapping functions during vegetative growth but only TUB1 is important for sexual reproduction. To better understand their functional divergence during ascos-porogenesis, in this study we characterized thesequence elements important for stage-specific func-tions of TUB1. Deletion of TUB1 blocked the late but not initial stages of perithecium formation. Perithecia formed by tub1 mutant had limited ascogenous hyphae and failed to develop asci. Silencing of TUB1 by MSUD also resulted in defects in ascospore formation. Interestingly, the 3 0 -UTR of TUB1 was dispensable for growth but essential for its function during sexual reproduction. RIP mutations that specifically affected Tub1 functions during sexual reproduction also were identified in two ascospore progeny. Furthermore, site-directed mutagenesis showed that whereas the non-editable mutations at three A-to-I RNA editing sites had no effects, the N347D (not T362D or I368V) edited mutation affected ascospore development. In addition, the F167Y, but not E198K or F200Y, mutation in TUB1 conferred tol-erance to carbendazim and caused a minor defect in sexual reproduction. Taken together, our data indi-cate TUB1 plays an essential role in ascosporogen-esis and sexual-specific functions of TUB1 require stage-specific RNA processing and Tub1 expression.