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Insights into the venom protein components of Microplitis mediator, an endoparasitoid wasp.

作者:   来源:   发布日期:2019-03-11  浏览次数:

  论文信息:Zhe Lin1, Rui-Juan Wang1, Yang Cheng, Jie Du, Olga Volovych, Li-Bin Han,Jian-Cheng Li, Yang Hu, Zi-Yun Lu, Zhiqiang Lu∗, Zhen Zou∗, Insights into the venom protein components of Microplitis mediator, an endoparasitoid wasp. Insect Biochemistry and Molecular Biology, 2019,105:33-42

  JCR分类:Q1区,昆虫学TOP期刊;中科院大类:2区; IF=3.562

  论文摘要:Endoparasitoid wasps deliver a variety of maternal factors, such as venom proteins, viruses, and virus-like particles, from their venom and calyx fluid into hosts and thereby regulate the hosts' immune response, metabolism and development. The endoparasitoid, Microplitis mediator, is used as an important biological agent for controlling the devastating pest Helicoverpa armigera. In this study, using an integrated transcriptomic and proteomic analysis approach, we identified 75 putative venom proteins in M. mediator. The identified venom components were consistent with other known parasitoid wasps’ venom proteins, including metalloproteases, serine protease inhibitors, and glycoside hydrolase family 18 enzymes. The metalloprotease and serpin family showed extensive gene duplications in venom apparatus. Isobaric tags for relative and absolute quantitation (iTRAQ) based quantitative proteomics revealed 521 proteins that were differentially expressed at 6 h and 24 h post-parasitism, including 10 wasp venom proteins that were released into the host hemolymph. Further analysis indicated that 511 differentially expressed proteins (DEP) from the host are primarily involved in the immune response, material metabolism, and extracellular matrix receptor interaction. Taken together, our results on parasitoid wasp venoms have the potential to enhance the application of endoparasitoid wasps for controlling insect pest
 

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